Preparation and Orientation of Canine Retinal Vasculature A Modified Trypsin Digestion Technique
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چکیده
منابع مشابه
HRP/trypsin technique for studies of the retinal vasculature.
The HRP/trypsin technique is a new histologic method for the light microscopic study of the retinal blood vessels. A two-stage procedure, the first step results in a retinal whole amount preparation which permits visualization of the three-dimensional architecture of perfused vessels and their relationship within the retina. This allows analysis of gross vessel morphology and differentiation of...
متن کاملComparison of retinal and cerebral vasculature in trypsin digest preparations.
The trypsin digestion technique for preparing flat mounts of the retinal capillary system was modified for comparable mounts of the cerebrocortical capillaries. These preparations, supplemented by electron microscopy, showed a basic similarity between the 2 systems, but the cortical capillaries have larger diameters, more abundant collateral channels, and less regularly spaced mural cells (intr...
متن کاملKinetics of Trypsin Digestion
The anomalies of enzyme action have frequently been accounted for by the assumption of an intermediate compound between the enzyme and the substrate. I t was shown, however, by Nelson and Hitchcock t that in the case of invertase the experiments could not be accurately calculated on this basis. The writer found with trypsin' that although the effect of varying the substrate concentration could ...
متن کاملThe kinetics of trypsin digestion.
The present study of a typical proteolytic enzyme extends the quantitative investigations previously reported from this laboratory on the effects of pressure on protein systems in viva (1) and in vitro (2). This paper presents new data on the self-digestion of trypsin and the digestion of casein by trypsin; a forthcoming paper will discuss the effects of temperature and hydrostatic pressure on ...
متن کامل240 Kinetics of Trypsin Digestion
The anomalies of enzyme action have frequently been accounted for by the assumption of an intermediate compound between the enzyme and the substrate. I t was shown, however, by Nelson and Hitchcock t that in the case of invertase the experiments could not be accurately calculated on this basis. The writer found with trypsin' that although the effect of varying the substrate concentration could ...
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ژورنال
عنوان ژورنال: Australian and New Zealand Journal of Ophthalmology
سال: 1978
ISSN: 0814-9763,1440-1606
DOI: 10.1111/j.1442-9071.1978.tb00252.x